DNA barcoding identification of Dermestidae species

Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4498-4502. doi: 10.3109/19401736.2015.1101538. Epub 2015 Nov 5.

Abstract

Dermestidae species are important stored product insects. The traditional morphological identification has its limitation. The purpose of the study is to explore the effectiveness of identification by DNA barcoding technology in Dermestidae. The COI gene sequences of 39 samples from 17 species of Dermestidae were analyzed, and we evaluated the identification ability of the sequences by the tree building method and the distance evaluation method in this study. The COI sequences whose length was 356 bp had a large difference between intra-specific and inter-specific and a significant barcoding gap. The success rate of identification was 92.5%. The results showed a certain feasibility to identify the most species of Dermestidae by this segment of COI.

Keywords: COI gene; DNA barcoding; Dermestidae; genetic distance; species identification.

MeSH terms

  • Animals
  • Coleoptera / classification
  • Coleoptera / genetics*
  • DNA / chemistry
  • DNA / isolation & purification
  • DNA / metabolism
  • DNA Barcoding, Taxonomic*
  • Databases, Genetic
  • Electron Transport Complex IV / classification
  • Electron Transport Complex IV / genetics
  • Electron Transport Complex IV / metabolism
  • Genetic Variation
  • Phylogeny
  • Sequence Analysis, DNA
  • Species Specificity

Substances

  • DNA
  • Electron Transport Complex IV