Efficacy and safety of the extracorporeal blood perfusion through DNase I- and C1q-containing magnetic beads have been evaluated using the experimental model simulating the nucleoprotein disposal disorders in systemic lupus erythematosus (SLE). The study was performed using 20 rats in which the essential impairments of nucleoprotein catabolism typical for SLE were modeled. The animals were randomized into the experimental group and the placebo perfusion control group. Rats of the experimental group were characterized by the statistically significant reduction of increased levels of circulating immune complexes and plasma DNA as well as diminished levels of plasma creatinine and kidney IgG deposition as compared with placebo controls. During short-term experiment there were neither animal deaths nor substantial blood cell destruction and hepatotoxicity signs.
Na éksperimental'noĭ modeli, vosproizvodiashcheĭ osnovnye narusheniia katabolizma nukleoproteinov pri sistemnoĭ krasnoĭ volchanke (SKV), izuchena éffektivnost' i bezopasnost' korrektsii dannykh rasstroĭstv s pomoshch'iu ékstrakorporal'noĭ perfuzii krovi cherez magnitoupravliaemye granuly, soderzhashchie DNKazu I i C1q. V éksperimente ispol'zovali 20 krys, kotorye byli randomizirovany v osnovnuiu podgruppu i podgruppu platsebo-kontrolia. V osnovnoĭ gruppe udalos' dostignut' statisticheski znachimogo snizheniia vysokikh urovneĭ tsirkuliruiushchikh immunnykh kompleksov i DNK plazmy, a takzhe dobit'sia dostovernogo umen'sheniia kontsentratsii kreatinina plazmy i soderzhaniia depozitov IgG v pochkakh po sravneniiu s platsebo-perfuzieĭ. V ramkakh ostrogo éksperimenta ne bylo otmecheno gibeli zhivotnykh, znachitel'noĭ destruktsii formennykh élementov i priznakov gepatotoksichnosti.
Keywords: C1q; DNase I; biosimulation; extracorporeal therapy; systemic lupus erythematosus.