Surface Modification of Polydivinylbenzene Microspheres with a Fluorinated Glycopolymer Using Thiol-Halogen Click Chemistry

Methods Mol Biol. 2016:1367:123-35. doi: 10.1007/978-1-4939-3130-9_10.

Abstract

Distillation-precipitation polymerization of divinylbenzene was applied to obtain uniform-sized polymeric microspheres. The microspheres were then modified with polypentafluorostyrene chains utilizing surface-initiated atom transfer radical polymerization techniques. The hydrophobic fluoropolymer-coated microsphere was then converted to a hydrophilic biopolymer by performing thiol-halogen click chemistry between polypentafluorostyrene and 1-thio-β-D-glucose sodium salt. The semi-fluorinated glycopolymer showed good binding ability with Concanavalin A as determined by confocal microscopy and turbidity experiments.

Keywords: Atom transfer radical polymerization; Glycopolymers; Polymeric microspheres; Protein binding; Surface modification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Click Chemistry
  • Concanavalin A / chemistry
  • Fluorine / chemistry
  • Glucose / analogs & derivatives*
  • Glucose / chemistry
  • Glycoconjugates / chemical synthesis*
  • Microspheres*
  • Polymers / chemical synthesis*
  • Polystyrenes / chemistry
  • Styrenes / chemical synthesis*
  • Styrenes / chemistry
  • Surface Properties

Substances

  • Glycoconjugates
  • Polymers
  • Polystyrenes
  • Styrenes
  • Concanavalin A
  • Fluorine
  • 1-thioglucose
  • poly(divinylbenzene)
  • Glucose