Detecting 16S rRNA Methyltransferases in Enterobacteriaceae by Use of Arbekacin

Patrick McGann; Sarah Chahine; Darius Okafor; Ana C Ong; Rosslyn Maybank; Yoon I Kwak; Kerry Wilson; Michael Zapor; Emil Lesho; Mary Hinkle

doi:10.1128/JCM.02642-15

Detecting 16S rRNA Methyltransferases in Enterobacteriaceae by Use of Arbekacin

J Clin Microbiol. 2016 Jan;54(1):208-11. doi: 10.1128/JCM.02642-15. Epub 2015 Nov 4.

Authors

Affiliations

¹ Multidrug-resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA patrick.t.mcgann4.ctr@mail.mil.
² Multidrug-resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
³ Infectious Diseases Service, Walter Reed National Military Medical Center, Bethesda, Maryland, USA.
⁴ Division of Bacterial & Rickettsial Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.

Abstract

16S rRNA methyltransferases confer resistance to most aminoglycosides, but discriminating their activity from that of aminoglycoside-modifying enzymes (AMEs) is challenging using phenotypic methods. We demonstrate that arbekacin, an aminoglycoside refractory to most AMEs, can rapidly detect 16S methyltransferase activity in Enterobacteriaceae with high specificity using the standard disk susceptibility test.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Anti-Infective Agents / metabolism*
Dibekacin / analogs & derivatives*
Dibekacin / metabolism
Disk Diffusion Antimicrobial Tests / methods*
Drug Resistance, Bacterial*
Enterobacteriaceae / drug effects*
Enterobacteriaceae / enzymology*
Genotype
Humans
Phenotype
RNA, Ribosomal, 16S / metabolism
tRNA Methyltransferases / analysis*
tRNA Methyltransferases / genetics

Substances

Anti-Infective Agents
RNA, Ribosomal, 16S
Dibekacin
tRNA Methyltransferases
arbekacin