The utilization of natural soda resource of Ordos in the cultivation of Nannochloropsis oceanica

Yanfei Pan; Haibo Yang; Yingying Meng; Jiao Liu; Peili Shen; Peichun Wu; Xupeng Cao; Song Xue

doi:10.1016/j.biortech.2015.10.068

The utilization of natural soda resource of Ordos in the cultivation of Nannochloropsis oceanica

Bioresour Technol. 2016 Jan:200:548-56. doi: 10.1016/j.biortech.2015.10.068. Epub 2015 Oct 27.

Authors

Yanfei Pan¹, Haibo Yang², Yingying Meng³, Jiao Liu⁴, Peili Shen⁴, Peichun Wu⁵, Xupeng Cao⁶, Song Xue⁵

Affiliations

¹ College of Environment and Chemical Engineering, Dalian University, Dalian 116622, China; Marine Bioengineering Group, Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, No. 457, Zhongshan Road, Dalian, China.
² College of Environment and Chemical Engineering, Dalian University, Dalian 116622, China.
³ Marine Bioengineering Group, Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, No. 457, Zhongshan Road, Dalian, China; School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
⁴ Marine Bioengineering Group, Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, No. 457, Zhongshan Road, Dalian, China; University of Chinese Academy of Sciences, Beijing 100049, China.
⁵ Marine Bioengineering Group, Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, No. 457, Zhongshan Road, Dalian, China.
⁶ Marine Bioengineering Group, Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, No. 457, Zhongshan Road, Dalian, China. Electronic address: c_x_p@dicp.ac.cn.

PMID: 26528905
DOI: 10.1016/j.biortech.2015.10.068

Abstract

Nannochloropsis oceanica is famous for its strong environmental adaptability and oil-richness, especially high eicosapentaenoic acid (EPA) content. In this report, the possibility and cultivation parameters for N. oceanica using natural crude soda were testified and compared with seawater culture. To keep a suitable salinity range, different ratio of sea salt addition into soda lake water were used and the growth, lipid content, Fv/Fm and fatty acids profiling were inspected with nitrogen repletion or depletion. The results showed the best performance were achieved while 18g/L (salinity 25‰) sea salt was added into crude soda solution. The μmax and EPA content in fatty acids were 0.72/0.42 and 36%/23% in 500mL/100L bioreactor cultivations respectively, which maintained a relative high productivity to other reports. By comparing the growth and operations with Spirulina production, the feasibility of N. Oceanica in Ordos was proved on both technical and economical point of view.

Keywords: EPA; Fatty acids; Nannochloropsis oceanica; Natural soda lake water; Ordos.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomass
Bioreactors*
Carbon / chemistry
Eicosapentaenoic Acid / biosynthesis*
Esters
Fatty Acids / biosynthesis*
Fatty Acids / chemistry
Fermentation
Lakes
Lipids / chemistry
Microalgae / growth & development
Nitrogen / chemistry*
Salinity
Salts
Seawater
Spirulina / growth & development
Stramenopiles / growth & development*

Substances

Esters
Fatty Acids
Lipids
Salts
Carbon
Eicosapentaenoic Acid
Nitrogen