Preparation of Urogenital Sinus Mesenchymal Cells for Prostate Tissue Recombination Models

Yang Zong; Andrew S Goldstein; Owen N Witte

doi:10.1101/pdb.prot078055

Preparation of Urogenital Sinus Mesenchymal Cells for Prostate Tissue Recombination Models

Cold Spring Harb Protoc. 2015 Nov 2;2015(11):988-90. doi: 10.1101/pdb.prot078055.

Authors

Yang Zong¹, Andrew S Goldstein², Owen N Witte³

Affiliations

¹ Howard Hughes Medical Institute, University of California, Los Angeles, California 90095;
² Department of Molecular and Medical Pharmacology, University of California, Los Angeles, California 90095; Department of Urology, University of California, Los Angeles, California 90095; Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, University of California, Los Angeles, California 90095; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, California 90095.
³ Howard Hughes Medical Institute, University of California, Los Angeles, California 90095; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, California 90095; Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, California 90095; Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, University of California, Los Angeles, California 90095; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, California 90095.

PMID: 26527759
DOI: 10.1101/pdb.prot078055

Abstract

An appropriate microenvironment provided by the mesenchyme is important for establishing tissue recombination models for epithelial cancer. Urogenital sinus mesenchymal (UGSM) cells derived from embryonic rodent show potent inductive effects for prostate regeneration. Genetic manipulation of these mesenchymal cells allows us to define the contribution of the tumor microenvironment to prostate cancer development. This protocol describes preparation and propagation of murine UGSM cells in culture.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Culture Techniques / methods*
Cell Separation / methods*
Mesenchymal Stem Cells / physiology*
Mice, Inbred C57BL
Urinary Bladder / cytology*
Urinary Bladder / embryology

Grants and funding

Howard Hughes Medical Institute/United States