Kinase phosphorylation monitoring with i-motif DNA cross-linked SERS probes

Chem Commun (Camb). 2016 Jan 7;52(2):410-3. doi: 10.1039/c5cc06566f. Epub 2015 Nov 3.

Abstract

We propose an ultrasensitive SERS-based peptide biosensor platform to monitor phosphorylation catalyzed by kinase in a dynamic format. The developed SERS strategy has a short response time with potential to monitor phosphorylation in live cells.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Biosensing Techniques / methods*
  • Cell Line, Tumor
  • DNA, Single-Stranded / chemistry
  • Gold / chemistry
  • Humans
  • Metal Nanoparticles / chemistry
  • Oligodeoxyribonucleotides / chemistry
  • Phosphorylation
  • Proto-Oncogene Proteins c-abl / chemistry*
  • Pyridines / chemistry
  • Spectrum Analysis, Raman

Substances

  • DNA, Single-Stranded
  • Oligodeoxyribonucleotides
  • Pyridines
  • 4-thiopyridine
  • Gold
  • Proto-Oncogene Proteins c-abl