Expression of HIP/PAP in hepatocellular carcinoma and effect of siRNA on migration and invasion in HCC cells

Gang Wang; Xin Zhao; De-Chun Li

doi:10.1016/j.apjtm.2015.09.012

Expression of HIP/PAP in hepatocellular carcinoma and effect of siRNA on migration and invasion in HCC cells

Asian Pac J Trop Med. 2015 Oct;8(10):848-54. doi: 10.1016/j.apjtm.2015.09.012. Epub 2015 Sep 25.

Authors

Gang Wang¹, Xin Zhao¹, De-Chun Li²

Affiliations

¹ Department of General Surgery, First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu, China.
² Department of General Surgery, First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu, China. Electronic address: lidechun2133@163.com.

PMID: 26522301
DOI: 10.1016/j.apjtm.2015.09.012

Abstract

Objective: To investigate the expression of HIP/PAP in hepatocellular carcinoma (HCC) patients and explore its role in migration and invasion of HCC.

Methods: The expression of HIP/PAP in HCC tissue and corresponding adjacent noncancerous tissue was assessed by IHC, RT-PCR and Western blot. The correlation between clinicopathological features and HIP/PAP expression was analyzed. The role of HIP/PAP on invasion and migration of HCC cells was observed by RNA interference, wound healing and Transwell assay.

Results: Both mRNA and protein expression of HIP/PAP was upregulated in HCC tissues compared to tumor-adjacent tissue and correlated with poor tumor differentiation, advanced tumor stage and vascular invasion. HIP/PAP expression was also upregulated in HCC cells, and silencing its expression by specific siRNA could inhibit the invasion and migration of HCC cells.

Conclusions: HIP/APA is overexpressed in HCC and contributes to the migration and invasion of HCC cells.

Keywords: HIP/PAP; Hepatocellular carcinoma; Invasion; Migration; Prognosis.