PKR-inhibitor binds efficiently with human microtubule affinity-regulating kinase 4

Farha Naz; Mohd Shahbaaz; Shama Khan; Krishna Bisetty; Asimul Islam; Faizan Ahmad; Md Imtaiyaz Hassan

doi:10.1016/j.jmgm.2015.10.009

PKR-inhibitor binds efficiently with human microtubule affinity-regulating kinase 4

J Mol Graph Model. 2015 Nov:62:245-252. doi: 10.1016/j.jmgm.2015.10.009. Epub 2015 Oct 21.

Authors

Farha Naz¹, Mohd Shahbaaz², Shama Khan¹, Krishna Bisetty², Asimul Islam¹, Faizan Ahmad¹, Md Imtaiyaz Hassan³

Affiliations

¹ Center for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India.
² Department of Chemistry, Durban University of Technology, Durban 4000, South Africa.
³ Center for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110025, India. Electronic address: mihassan@jmi.ac.in.

PMID: 26519933
DOI: 10.1016/j.jmgm.2015.10.009

Abstract

MAP/microtubule affinity-regulating kinase 4 (MARK4) plays a central role in the cellular physiology, and it is inseparably linked with many human diseases including cancer, diet induced obesity, type2 diabetes and neurodegenerative disorders. Here, we studied the interaction of PKR-inhibitor with two variants of human MARK4. One variant is named as MARK4-F1 which has 59 N-terminal residues along with kinase domain while another variant is MARK4-F2 which has kinase domain only. Molecular-docking, molecular dynamics (MD) simulation and fluorescence-binding studies were undertaken to understand the role of N-terminal 59-residues in the binding of substrate/inhibitors. Molecular docking studies revealed that the PKR-inhibitor binds in the large hydrophobic cavity of the kinase domain of MARK4 through several hydrophobic and hydrogen-bonded interactions. Furthermore, MD simulation showed a stable parameters for the complexes of both MARK4-F1 and MARK4-F2 to PKR-inhibitor with marginal difference in their binding affinities. A significant decrease in the fluorescence intensity of MARK4 was observed on successive addition of the PKR-inhibitor. Using fluorescence data we have calculated the binding-affinity and the number of binding site of PKR-inhibitor to the MARK4. A significantly high binding affinity was observed for the PKR-inhibitor to the MARK4 variants. However, there is no any significant difference in the binding affinity of PKR-inhibitor to the MARK4 variants was observed, indicating that 59 N-terminal residues of MARK4-F1 are not playing a crucial role in the ligand binding. The present study will provide an insights into designing of new PKR-inhibitor derivative as potent and selective therapeutic agent against many life threatening diseases which are associated with MARK4.

Keywords: Docking; Drug target; High affinity ligand; MAP/microtubule affinity-regulating kinase 4; Molecular dynamics simulation; PKR-inhibitor.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Binding Sites
Humans
Molecular Docking Simulation
Molecular Dynamics Simulation
Protein Binding
Protein Interaction Domains and Motifs
Protein Kinase Inhibitors / chemistry*
Protein Serine-Threonine Kinases / antagonists & inhibitors
Protein Serine-Threonine Kinases / chemistry*

Substances

Protein Kinase Inhibitors
MARK4 protein, human
Protein Serine-Threonine Kinases