Fission Yeast Cell Cycle Synchronization Methods

Marta Tormos-Pérez; Livia Pérez-Hidalgo; Sergio Moreno

doi:10.1007/978-1-4939-3145-3_20

Fission Yeast Cell Cycle Synchronization Methods

Methods Mol Biol. 2016:1369:293-308. doi: 10.1007/978-1-4939-3145-3_20.

Authors

Marta Tormos-Pérez^{1

2}, Livia Pérez-Hidalgo^{1

2}, Sergio Moreno^{3

4}

Affiliations

¹ Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas/Universidad de Salamanca, 37007, Salamanca, Spain.
² Instituto de Investigación Biomédica de Salamanca (IBSAL), 37007, Salamanca, Spain.
³ Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas/Universidad de Salamanca, 37007, Salamanca, Spain. smo@usal.es.
⁴ Instituto de Investigación Biomédica de Salamanca (IBSAL), 37007, Salamanca, Spain. smo@usal.es.

PMID: 26519320
DOI: 10.1007/978-1-4939-3145-3_20

Abstract

Fission yeast cells can be synchronized by cell cycle arrest and release or by size selection. Cell cycle arrest synchronization is based on the block and release of temperature-sensitive cell cycle mutants or treatment with drugs. The most widely used approaches are cdc10-129 for G1; hydroxyurea (HU) for early S-phase; cdc25-22 for G2, and nda3-KM311 for mitosis. Cells can also be synchronized by size selection using centrifugal elutriation or a lactose gradient. Here we describe the methods most commonly used to synchronize fission yeast cells.

Keywords: Block and release; Centrifugal elutriation; Fission yeast; G1-phase; G2-phase; HU; Lactose gradient; Mitosis; S-phase; Schizosaccharomyces pombe; Synchronization; cdc10-129; cdc25-22; nda3-KM311.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Cycle* / drug effects
Cell Cycle* / genetics
Hydroxyurea / pharmacology
Mitosis / drug effects
Mitosis / genetics
Mutation
Schizosaccharomyces / physiology*

Substances

Hydroxyurea