Abstract
Islet amyloid polypeptide (IAPP) is the 37-amino acid peptide subunit of amyloid found in pancreatic islets of type 2 diabetic patients and in insulinomas. Recently, we isolated the human gene encoding IAPP [(1988) FEBS Lett. 239, 227-232]. We now report the nucleotide sequences of a human insulinoma cDNA encoding a complete IAPP precursor, and of the corresponding parts of the IAPP gene. Two exons, which are approx. 5 kb apart in the human genome, encode the 89-amino acid pre-pro-IAPP. At least one additional exon is present further upstream in the IAPP gene. A putative signal sequence at the amino-terminus of the precursor suggests that IAPP is a secreted protein.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Amyloid / genetics*
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Base Sequence
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Calcitonin / genetics
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Calcitonin Gene-Related Peptide
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Codon
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DNA / genetics
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DNA Probes
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Exons*
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Humans
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Insulinoma / analysis
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Islet Amyloid Polypeptide
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Islets of Langerhans / analysis*
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Molecular Sequence Data
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Neuropeptides / genetics
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Nucleic Acid Hybridization
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Pancreatic Neoplasms / analysis
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Protein Precursors / genetics*
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Protein Sorting Signals / genetics
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Sequence Homology, Nucleic Acid
Substances
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Amyloid
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Codon
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DNA Probes
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Islet Amyloid Polypeptide
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Neuropeptides
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Protein Precursors
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Protein Sorting Signals
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Calcitonin
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DNA
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Calcitonin Gene-Related Peptide
Associated data
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GENBANK/X14902
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GENBANK/X14903
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GENBANK/X14904
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GENBANK/X14905
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GENBANK/Y07495