Monitoring photodynamic therapy with photoacoustic microscopy

Peng Shao; David W Chapman; Ronald B Moore; Roger J Zemp

doi:10.1117/1.JBO.20.10.106012

Monitoring photodynamic therapy with photoacoustic microscopy

J Biomed Opt. 2015 Oct;20(10):106012. doi: 10.1117/1.JBO.20.10.106012.

Authors

Peng Shao¹, David W Chapman², Ronald B Moore², Roger J Zemp¹

Affiliations

¹ University of Alberta, Department of Electrical & Computer Engineering, 9107-116 Street, Edmonton T6G 2V4, Canada.
² University of Alberta, Department of Surgery and Oncology, 11560 University Avenue, Edmonton T6G 1Z2, Canada.

PMID: 26509414
DOI: 10.1117/1.JBO.20.10.106012

Abstract

Abstract. We present our work on examining the feasibility of monitoring photodynamic therapy (PDT)-induced vasculature change with acoustic-resolution photoacoustic microscopy (PAM). Verteporfin, an FDA-approved photosensitizer for clinical PDT, was utilized. With a 60-μm-resolution PAM system, we demonstrated the capability of PAM to monitor PDT-induced vasculature variations in a chick chorioallantoic membrane model with topical application and in a rat ear with intravenous injection of the photosensitizer. We also showed oxygen saturation change in target blood vessels due to PDT. Success of the present approach may potentially lead to the application of PAM imaging in evaluating PDT efficacy, guiding treatment, and predicting responders from nonresponders.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Drug Monitoring / instrumentation
Drug Monitoring / methods*
Equipment Design
Equipment Failure Analysis
Feasibility Studies
Microscopy, Acoustic / instrumentation
Microscopy, Acoustic / methods*
Photoacoustic Techniques / instrumentation
Photoacoustic Techniques / methods*
Photochemotherapy / methods*
Reproducibility of Results
Sensitivity and Specificity
Treatment Outcome