Pyrazinamide resistance determined by liquid culture at low pH better correlates with genetic mutations in MDR tuberculosis isolates

Yu Pang; Zhongdong Wang; Huiwen Zheng; Yuanyuan Song; Yufeng Wang; Yanlin Zhao

doi:10.1016/j.mimet.2015.10.014

Pyrazinamide resistance determined by liquid culture at low pH better correlates with genetic mutations in MDR tuberculosis isolates

J Microbiol Methods. 2015 Dec:119:142-4. doi: 10.1016/j.mimet.2015.10.014. Epub 2015 Oct 23.

Authors

Yu Pang¹, Zhongdong Wang², Huiwen Zheng³, Yuanyuan Song³, Yufeng Wang³, Yanlin Zhao⁴

Affiliations

¹ National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China. Electronic address: pangyu@chinatb.org.
² Qingdao Center for Disease Control and Prevention, Qingdao, Shangdong, China.
³ National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
⁴ National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China. Electronic address: zhaoyanlin@chinatb.org.

PMID: 26506283
DOI: 10.1016/j.mimet.2015.10.014

Abstract

We detected the pyrazinamide (PZA) susceptibility of Mycobacterium tuberculosis strains in the Bactec MGIT 960 liquid medium with different pH values. Our results demonstrated that PZA resistance determined by liquid culture at pH 5.7 (94.9%) showed better correlation with genetic changes among multidrug-resistant M. tuberculosis isolates (P=0.001).

Keywords: Drug resistance; MGIT; Mycobacterium tuberculosis; Pyrazinamide.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antitubercular Agents / pharmacology*
Hydrogen-Ion Concentration
Microbial Sensitivity Tests
Mycobacterium tuberculosis / drug effects*
Mycobacterium tuberculosis / genetics
Pyrazinamide / pharmacology*
Tuberculosis, Multidrug-Resistant / microbiology*

Substances

Antitubercular Agents
Pyrazinamide