Emergence of Mutations in the K13 Propeller Gene of Plasmodium falciparum Isolates from Dakar, Senegal, in 2013-2014

Antimicrob Agents Chemother. 2015 Oct 26;60(1):624-7. doi: 10.1128/AAC.01346-15. Print 2016 Jan.

Abstract

The kelch 13 (K13) propeller gene is associated with artemisinin resistance. In a previous work, there were no mutations found in 138 Plasmodium falciparum isolates collected in 2012 and 2013 from patients residing in Dakar, Senegal (M. Torrentino-Madamet et al., Malar J 13:472, 2014, http://dx.doi.org/10.1186/1475-2875-13-472). However, the N554H, Q613H, and V637I mutations were identified in the propeller region of K13 in 92 (5.5%) isolates in 2013 and 2014. There were five polymorphisms identified in the Plasmodium/Apicomplexa-specific domain (K123R, N137S, N142NN/NNN, T149S, and K189T/N).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antimalarials / pharmacology
  • Artemisinins / pharmacology
  • Drug Resistance / genetics
  • Gene Expression
  • Humans
  • Malaria, Falciparum / drug therapy
  • Malaria, Falciparum / epidemiology
  • Malaria, Falciparum / parasitology
  • Microfilament Proteins / chemistry
  • Microfilament Proteins / genetics*
  • Microfilament Proteins / metabolism
  • Models, Molecular
  • Mutation*
  • Plasmodium falciparum / drug effects
  • Plasmodium falciparum / genetics*
  • Plasmodium falciparum / growth & development
  • Plasmodium falciparum / metabolism
  • Polymorphism, Genetic
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Protozoan Proteins / chemistry
  • Protozoan Proteins / genetics*
  • Protozoan Proteins / metabolism
  • Senegal / epidemiology

Substances

  • Antimalarials
  • Artemisinins
  • Microfilament Proteins
  • Protozoan Proteins
  • artemisinin

Grants and funding

This research was supported by the Schéma directeur Paludisme, Etat Major des Armées Françaises (grant LR 607a), and by the Ministère des Affaires Etrangères.