Metabolomics of Daucus carota cultured cell lines under stressing conditions reveals interactions between phenolic compounds

Stefania Ceoldo; Ketti Toffali; Silvia Mantovani; Greta Baldan; Marisa Levi; Flavia Guzzo

doi:10.1016/j.plantsci.2009.01.011

Metabolomics of Daucus carota cultured cell lines under stressing conditions reveals interactions between phenolic compounds

Plant Sci. 2009 Apr;176(4):553-65. doi: 10.1016/j.plantsci.2009.01.011. Epub 2009 Jan 30.

Authors

Stefania Ceoldo¹, Ketti Toffali¹, Silvia Mantovani¹, Greta Baldan¹, Marisa Levi¹, Flavia Guzzo²

Affiliations

¹ University of Verona, Science and Technology Department, Strada le Grazie 15, Cà Vignal 1, 37134 Verona, Italy.
² University of Verona, Science and Technology Department, Strada le Grazie 15, Cà Vignal 1, 37134 Verona, Italy. Electronic address: flavia.guzzo@univr.it.

PMID: 26493146
DOI: 10.1016/j.plantsci.2009.01.011

Abstract

A metabolomic approach followed by principal components and partial least square analysis was used for investigating the effect of environmental factors on two Daucus carota L. cv. Flakkese cell lines (R3M and R4G), selected for their ability to produce anthocyanins in the light and the dark, respectively. A positive correlation between total anthocyanin, hydroxycinnamic and hydroxybenzoic acid accumulation was found in both lines. Furthermore, the experimental design and the combination of biochemical and statistical analyses allowed us to unravel complex relationships between environmental factors and phenylpropanoid composition. Among these, the induction by mechanical stress of overproduction of all anthocyanins, hydroxycinnamic and hydroxybenzoic acids except sinapic acid derivatives, whose accumulation was inhibited.

Keywords: Anthocyanins; Daucus carota; Hydroxybenzoic acids; Hydroxycinnamic acids; Metabolomics.