Abstract
IL-17 is an ancient cytokine implicated in a variety of immune defense reactions. We identified five members of the sea lamprey IL-17 family (IL-17D.1, IL-17D.2, IL-17E, IL-17B, and IL-17C) and six IL-17R genes (IL-17RA.1, IL-17RA.2, IL-17RA.3, IL-17RF, IL-17RE/RC, and IL-17RD), determined their relationship with mammalian orthologs, and examined their expression patterns and potential interactions to explore their roles in innate and adaptive immunity. The most highly expressed IL-17 family member is IL-17D.1 (mammalian IL-17D like), which was found to be preferentially expressed by epithelial cells of skin, intestine, and gills and by the two types of lamprey T-like cells. IL-17D.1 binding to rIL-17RA.1 and to the surface of IL-17RA.1-expressing B-like cells and monocytes of lamprey larvae was demonstrated, and treatment of lamprey blood cells with rIL-17D.1 protein enhanced transcription of genes expressed by the B-like cells. These findings suggest a potential role for IL-17 in coordinating the interactions between T-like cells and other cells of the adaptive and innate immune systems in jawless vertebrates.
Copyright © 2015 by The American Association of Immunologists, Inc.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptive Immunity / immunology
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal / immunology
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B-Lymphocytes / immunology*
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Cell Line
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Epithelial Cells / metabolism
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Gills / metabolism
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HEK293 Cells
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Humans
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Immunity, Innate / immunology
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Interleukin-17 / genetics*
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Interleukin-17 / immunology
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Interleukin-17 / metabolism
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Interleukin-27 / genetics*
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Interleukin-27 / immunology
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Interleukin-27 / metabolism
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Intestinal Mucosa / metabolism
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Petromyzon / immunology*
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Phylogeny
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Protein Binding / immunology
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RNA, Messenger / genetics
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Receptors, Interleukin-17 / biosynthesis
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Receptors, Interleukin-17 / genetics*
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Receptors, Interleukin-17 / metabolism
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Skin / cytology
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Skin / metabolism
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T-Lymphocytes / immunology*
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Transcriptome / genetics
Substances
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Antibodies, Monoclonal
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Interleukin-17
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Interleukin-27
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RNA, Messenger
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Receptors, Interleukin-17
Associated data
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GENBANK/KR059941
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GENBANK/KR059942
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GENBANK/KR059943
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GENBANK/KR059944
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GENBANK/KR059945
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GENBANK/KR059946
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GENBANK/KR059947
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GENBANK/KR059948
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GENBANK/KR059949
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GENBANK/KR059950
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GENBANK/KR059951
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GENBANK/KR059952
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GENBANK/KR059953
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GENBANK/KR059954
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GENBANK/KR059955
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GENBANK/KR059956