Immobilization of peptides to a solid surface is frequently an important first step before they can be probed with a variety of biological samples in a heterogeneous assay format for research and clinical diagnostic purposes. Peptides can be derivatized in many ways to subsequently covalently attach them to an activated solid surface such as epoxy-functionalized glass slides. Here, we describe a clean, efficient, and reproducible fabrication process based on catalyst-free click chemistry compatible with the construction of low- to high-density peptide microarrays.
Keywords: Azadibenzocyclooctyne (ADIBO ); Catalyst-free; Click chemistry; Immunoassays; Peptide microarrays; Strain-promoted alkyne-azide cycloaddition (SPAAC ); c-Myc peptide.