High-Throughput Peptide Screening on a Bimodal Imprinting Chip Through MS-SPRi Integration

Methods Mol Biol. 2016:1352:111-25. doi: 10.1007/978-1-4939-3037-1_9.

Abstract

Screening of high affinity and high specificity peptide probes towards various targets is important in the biomedical field while traditional peptide screening procedure is manual and tedious. Herein, a bimodal imprinting microarray system to embrace the whole peptide screening process is presented. Surface Plasmon Resonance imaging (SPRi) and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) are combined for both quantitative and qualitative identification of the peptide. The method provides a solution for high efficiency peptide screening.

Keywords: Bimodal imprinting chip; MALDI-TOF-MS; Microarray; OBOC; SPRi.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Biotinylation
  • Hemagglutinin Glycoproteins, Influenza Virus / chemistry
  • Peptides / chemical synthesis
  • Peptides / chemistry
  • Peptides / metabolism*
  • Protein Array Analysis / methods*
  • Solid-Phase Synthesis Techniques
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Surface Plasmon Resonance / methods*
  • Systems Integration*

Substances

  • Hemagglutinin Glycoproteins, Influenza Virus
  • Peptides