Analytical sequence to study G-CSF effect on the transcriptome of isolated spinal motoneurons from SOD1 G93A mice, an animal model for amyotrophic lateral sclerosis

Alexandre Henriques; Stefan Kastner; Oliver Wafzig; Jose-Luis Gonzalez De Aguilar; Armin Schneider

doi:10.1016/j.gdata.2015.02.003

Analytical sequence to study G-CSF effect on the transcriptome of isolated spinal motoneurons from SOD1 G93A mice, an animal model for amyotrophic lateral sclerosis

Genom Data. 2015 Feb 28:4:47-9. doi: 10.1016/j.gdata.2015.02.003. eCollection 2015 Jun.

Authors

Alexandre Henriques¹, Stefan Kastner², Oliver Wafzig², Jose-Luis Gonzalez De Aguilar¹, Armin Schneider²

Affiliations

¹ INSERM, U1118, Mécanismes Centraux et Péripheriques de la Neurodégénérescence, Strasbourg, France ; UMRS1118, Fédération de Médecine Translationnelle de Strasbourg, Université de Strasbourg, France.
² Sygnis Bioscience GmbH & Co KG, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany.

Abstract

Granulocyte-colony stimulating factor (G-CSF) has been recently identified as a neurotrophic factor able to preserve motor functions, rescue motor units and extent survival in an animal model of amyotrophic lateral sclerosis, the SOD1 G93A mice. To gain insight into the mode of action of G-CSF, we have recently performed gene expression profiling on isolated lumbar motoneurons from SOD1G93A mice, and shown that G-CSF re-adjusted gene expression in motoneurons of symptomatic SOD1G93A mice and modulates genes related to neuromuscular function (Henriques et al., 2015). Here, we provide quality controls for the microarray experiment (GO accession number GSE60856) and describe the experimental strategy.

Keywords: ALS; G-CSF; Gene expression; Laser microdissection; Motor neuron.