The high-throughput sequencing of small RNAs profiling in wide hybridisation and allopolyploidisation between Brassica rapa and Brassica nigra

Muhammad Awais Ghani; Junxing Li; Linli Rao; Muhammad Ammar Raza; Liwen Cao; Ningning Yu; Xiaoxia Zou; Liping Chen

doi:10.1016/j.gdata.2014.10.021

The high-throughput sequencing of small RNAs profiling in wide hybridisation and allopolyploidisation between Brassica rapa and Brassica nigra

Genom Data. 2014 Nov 8:3:1-3. doi: 10.1016/j.gdata.2014.10.021. eCollection 2015 Mar.

Authors

Muhammad Awais Ghani¹, Junxing Li¹, Linli Rao¹, Muhammad Ammar Raza¹, Liwen Cao¹, Ningning Yu¹, Xiaoxia Zou¹, Liping Chen¹

Affiliation

¹ Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Yuhangtang Road No. 866, Hangzhou 310058, Zhejiang Province, PR China.

Abstract

Small RNAs play an important role in maintaining the genome reconstruction and stability in the plant. However, little is known regarding the role of small RNAs during the process of wide hybridisation and chromosome doubling. Therefore, the changes in the small RNAs were assessed during the formation of an allodiploid (genome: AB) and its allotetraploid (genome: AABB) between Brassica rapa (♀) and Brassica nigra (♂) in the present study. Here, the experimental methods described in details, RNA-seq data (available at Gene Expression Omnibus database under GSE61872) and analysis published by Ghani et al. [1]. The study showed that small RNAs play an important role in maintaining the genome stability, and regulate gene expression which induces the phenotype variation in the formation of an allotetraploid. This may play an important role in the occurrence of heterosis in the allotetraploid.

Keywords: Allopolyploidisation; Small RNAs; Wide hybridisation.