Development and Application of Spin Traps, Spin Probes, and Spin Labels

Elena G Bagryanskaya; Olesya A Krumkacheva; Matvey V Fedin; Sylvain R A Marque

doi:10.1016/bs.mie.2015.06.004

Development and Application of Spin Traps, Spin Probes, and Spin Labels

Methods Enzymol. 2015:563:365-96. doi: 10.1016/bs.mie.2015.06.004. Epub 2015 Jul 2.

Authors

Elena G Bagryanskaya¹, Olesya A Krumkacheva², Matvey V Fedin², Sylvain R A Marque³

Affiliations

¹ N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry SB RAS, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia. Electronic address: egbagryanskaya@nioch.nsc.ru.
² Novosibirsk State University, Novosibirsk, Russia; International Tomography Center SB RAS, Novosibirsk, Russia.
³ Aix-Marseille Université, Marseille Cedex, France.

PMID: 26478492
DOI: 10.1016/bs.mie.2015.06.004

Abstract

This chapter focuses on major achievements of the last decade in the synthesis and applications of spin traps, spin probes, and spin labels. Our discussion on spin trapping is mainly concerned with novel aspects of nitrones used as spin traps and with the kinetics caused by bioreductants. The second part of the chapter deals with recent developments in site-directed spin labeling (SDSL) for studying structure and functions of proteins and nucleic acids. We focus on SDSL EPR distance measurements using advanced trityl and nitroxide labels, on new approaches for incorporation of spin labels in biomolecules, and finally, on recent room/physiological temperature measurements made feasible by these novel spin labels.

Keywords: DNA; DQC; EPR; Nitrones; Nitroxide; Organic synthesis; PELDOR/DEER; Spin labels; Spin traps; Trityl radicals.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Electron Spin Resonance Spectroscopy / methods*
Nitrogen Oxides / chemistry
Nucleic Acids / chemistry*
Proteins / chemistry*
Spin Labels / chemical synthesis*

Substances

Nitrogen Oxides
Nucleic Acids
Proteins
Spin Labels
nitrones