An optimised perfusion technique for extracting murine gastric leukocytes

Garrett Z Ng; Philip Sutton

doi:10.1016/j.jim.2015.10.004

An optimised perfusion technique for extracting murine gastric leukocytes

J Immunol Methods. 2015 Dec:427:126-9. doi: 10.1016/j.jim.2015.10.004. Epub 2015 Oct 22.

Authors

Garrett Z Ng¹, Philip Sutton²

Affiliations

¹ Mucosal Immunology, Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia. Electronic address: garrett.ng@mcri.edu.au.
² Mucosal Immunology, Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia; Centre for Animal Biotechnology, School of Veterinary and Agricultural Science, University of Melbourne, Parkville, Victoria, Australia.

PMID: 26478184
DOI: 10.1016/j.jim.2015.10.004

Abstract

The stomach is a difficult tissue to analyse by flow cytometry, largely due to the difficulty of isolating viable leukocytes. Here we present the re-optimization of a perfusion technique that compares favourably against two other methods of enzymatic digestion for the release of gastric leukocytes. We believe that this technique could greatly assist the analysis of immune cells basally present in the murine stomach and that infiltrate during infection or disease.

Keywords: Flow cytometry; Leukocytes; Stomach.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Flow Cytometry / methods*
Leukocytes / cytology*
Leukocytes / immunology
Mice
Mice, Inbred C57BL
Mice, Inbred NOD
Mice, SCID
Perfusion / methods*
Stomach / cytology*
Stomach / immunology*