Sesquiterpenoids from Tussilago farfara inhibit LPS-induced nitric oxide production in macrophage RAW 264.7 cells

Arch Pharm Res. 2016 Jan;39(1):127-32. doi: 10.1007/s12272-015-0667-7. Epub 2015 Oct 17.

Abstract

A new oplopane-type sesquiterpenoid tussilagofarin (1) and a new chromone tussilagofarol (2), along with 18 known compounds 3-20, were isolated from the flower buds of Tussilago farfara. The structures of the new compounds were elucidated on the basis of 1D and 2D NMR and HRESIMS data. Of the isolated compounds, oplopane- and bisabolane-type sesquterpenoids 1, 8-12, 15, and 16 were found to inhibit nitric oxide production in LPS-induced RAW 264.7 cells with IC50 values of 3.5-28.5 μM.

Keywords: Asteraceae; Nitric oxide production inhibitor; Oplopane-type sesquiterpenoid; Tussilago farfara.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Flowers
  • Lipopolysaccharides / antagonists & inhibitors*
  • Lipopolysaccharides / toxicity
  • Macrophages / drug effects*
  • Macrophages / metabolism
  • Mice
  • Nitric Oxide / antagonists & inhibitors*
  • Nitric Oxide / biosynthesis
  • RAW 264.7 Cells
  • Sesquiterpenes / isolation & purification*
  • Sesquiterpenes / pharmacology*
  • Tussilago*

Substances

  • Lipopolysaccharides
  • Sesquiterpenes
  • Nitric Oxide