Development of a Sensitive Bioluminogenic Probe for Imaging Highly Reactive Oxygen Species in Living Rats

Angew Chem Int Ed Engl. 2015 Dec 1;54(49):14768-71. doi: 10.1002/anie.201507530. Epub 2015 Oct 16.

Abstract

A sensitive bioluminogenic probe for highly reactive oxygen species (hROS), SO3 H-APL, was developed based on the concept of dual control of bioluminescence emission by means of bioluminescent enzyme-induced electron transfer (BioLeT) and modulation of cell-membrane permeability. This probe enables non-invasive visualization of physiologically relevant amounts of hROS generated deep inside the body of living rats for the first time. It is expected to serve as a practical analytical tool for investigating a wide range of biological functions of hROS in vivo. The design concept should be applicable to other in vivo bioluminogenic probes.

Keywords: electron transfer; imaging agents; luminescence; membrane permeability; reactive oxygen species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Luciferases / genetics
  • Luciferases / metabolism
  • Luminescent Agents / administration & dosage
  • Luminescent Agents / analysis*
  • Luminescent Agents / chemical synthesis*
  • Luminescent Agents / chemistry
  • Luminescent Measurements
  • Molecular Structure
  • Rats
  • Rats, Transgenic
  • Reactive Oxygen Species / analysis*
  • Reactive Oxygen Species / metabolism*

Substances

  • Luminescent Agents
  • Reactive Oxygen Species
  • Luciferases