AIM. Study the effect of fluconazole antimycotic on temporal organization of biological properties of Candida albicans.
Materials and methods: Clinical isolates and a museum C. albicans strain were used. The study was carried out for 24 hours with a 4 hour interval, determining proliferative, adhesive, morphological, protease, catalase, phospholipase activity. The results of the study were treated statistically.
Results: Maximal sensitivity to fluconazole was established for the museum C. albicans strain during the day, in clinical isolates--in the evening and at night. After the effect of 1/2 minimal inhibitory concentration (MIC) of the preparation, the rhythms of all the parameters changed in the museum culture from circadian to ultradian: The preparation reduced mesor and amplitude of the oscillation of proliferative and adhesive activity, but did not have an effect on chrono-infrastructure of morphogenesis and aggression enzymes. The temporal rows of biological activity of pathogenic C. albicans isolates after the incubation with fluconazole were characterized by synchronization of all the properties in a narrow temporal interval from 07:00 to 11:00 and from 16:00 to 23:00 hours.
Conclusion: The results obtained give evidence on the lability of adhesion factors and colonization and stability of virulence factors in the museum variant. A clear synchronization in time of all the pathogenicity factors of clinical isolates gives evidence on the economy of material and energy resources, opening a perspective of using the chronobiological approach in antibiotics therapy of candidoses.