Comparative Evaluation of AmpliVue HSV 1+2 Assay with ELVIS Culture for Detecting Herpes Simplex Virus 1 (HSV-1) and HSV-2 in Clinical Specimens

J Clin Microbiol. 2015 Dec;53(12):3922-5. doi: 10.1128/JCM.01905-15. Epub 2015 Oct 14.

Abstract

The AmpliVue HSV 1+2 assay was compared to the ELVIS HSV ID and D(3) Typing Culture System for the qualitative detection and differentiation of herpes simplex virus 1 (HSV-1) and HSV-2 DNA in 1,351 cutaneous and mucocutaneous specimens. Compared to ELVIS, AmpliVue had sensitivities of 95.7 and 97.6% for detecting HSV-1 and HSV-2, respectively. Following arbitration of discordant results by an independent molecular method, the AmpliVue assay had a resolved sensitivity and specificity of 99.2 and 99.7%, respectively, for both HSV-1 and HSV-2, whereas ELVIS had a resolved sensitivity of 87.1% for HSV-1 and 84.5% for HSV-2.

Publication types

  • Comparative Study
  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Herpes Simplex / diagnosis*
  • Herpes Simplex / virology
  • Herpesvirus 1, Human / isolation & purification*
  • Herpesvirus 2, Human / isolation & purification*
  • Humans
  • Molecular Diagnostic Techniques / methods*
  • Mucous Membrane / virology*
  • Sensitivity and Specificity
  • Skin / virology*
  • Virus Cultivation / methods*