Quantitative Measurements of Type I and Type II Persisters Using ScanLag

Irit Levin-Reisman; Nathalie Q Balaban

doi:10.1007/978-1-4939-2854-5_7

Quantitative Measurements of Type I and Type II Persisters Using ScanLag

Methods Mol Biol. 2016:1333:75-81. doi: 10.1007/978-1-4939-2854-5_7.

Authors

Irit Levin-Reisman¹, Nathalie Q Balaban²

Affiliations

¹ The Racah Institute of Physics, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel.
² The Racah Institute of Physics, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel. nathalieqb@phys.huji.ac.il.

PMID: 26468101
DOI: 10.1007/978-1-4939-2854-5_7

Abstract

The present method quantifies the number of slow-growing bacteria leading to antibiotic persistence in a clonal population. First, it enables discriminating between slow growers that are generated by exposure to a stress signal (Type I persisters) and slow growers that are continuously generated during exponential growth (Type II persisters). Second, the method enables determining the amount of slow growers in a culture.

Keywords: Automatic imaging; Bacterial growth; High-throughput measurements; Lag; Persisters; ScanLag; Stationary phase; Type I persistence; Type II persistence.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology*
Cell Culture Techniques / methods*
Drug Resistance, Bacterial / drug effects
Drug Resistance, Bacterial / genetics*
Escherichia coli / drug effects*
Escherichia coli / growth & development
Humans

Substances

Anti-Bacterial Agents