A molecular survey of Eimeria in chickens across Australia

Rosamond M Godwin; Jess A T Morgan

doi:10.1016/j.vetpar.2015.09.030

A molecular survey of Eimeria in chickens across Australia

Vet Parasitol. 2015 Nov 30;214(1-2):16-21. doi: 10.1016/j.vetpar.2015.09.030. Epub 2015 Oct 1.

Authors

Rosamond M Godwin¹, Jess A T Morgan²

Affiliations

¹ Agri-Science Queensland, Department of Agriculture and Fisheries, St. Lucia, QLD 4067, Australia; Poultry CRC, PO Box U242, University of New England, Armidale, NSW 2351, Australia.
² Agri-Science Queensland, Department of Agriculture and Fisheries, St. Lucia, QLD 4067, Australia; Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, PO Box 6097, St. Lucia, QLD 4067, Australia. Electronic address: jessica.morgan@uq.edu.au.

PMID: 26467277
DOI: 10.1016/j.vetpar.2015.09.030

Abstract

Coccidiosis is a costly enteric disease of chickens caused by protozoan parasites of the genus Eimeria. Disease diagnosis and management is complicated since there are multiple Eimeria species infecting chickens and mixed species infections are common. Current control measures are only partially effective and this, combined with concerns over vaccine efficacy and increasing drug resistance, demonstrates a need for improved coccidiosis diagnosis and control. Before improvements can be made, it is important to understand the species commonly infecting poultry flocks in both backyard and commercial enterprises. The aim of this project was to conduct a survey and assessment of poultry Eimeria across Australia using genetic markers, and create a collection of isolates for each Eimeria species. A total of 260 samples (faecal or caecal) was obtained, and survey results showed that Eimeria taxa were present in 98% of commercial and 81% of backyard flocks. The distribution of each Eimeria species was widespread across Australia, with representatives of all species being found in every state and territory, and the Eimeria species predominating in commercial flocks differed from those in backyard flocks. Three operational taxonomic units also occurred frequently in commercial flocks highlighting the need to understand the impact of these uncharacterised species on poultry production. As Eimeria infections were also frequent in backyard flocks, there is a potential for backyard flocks to act as reservoirs for disease, especially as the industry moves towards free range production systems. This Eimeria collection will be an important genetic resource which is the crucial first step in the development of more sophisticated diagnostic tools and the development of new live vaccines which ultimately will provide savings to the industry in terms of more efficient coccidiosis management.

Keywords: Capillary electrophoresis; Eimeria; Mitochondrial DNA; PCR; Species survey.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Australia / epidemiology
Chickens*
Coccidiosis / epidemiology
Coccidiosis / parasitology
Coccidiosis / veterinary*
Eimeria / classification
Eimeria / isolation & purification*
Poultry Diseases / epidemiology
Poultry Diseases / parasitology*