The implementation of the National Policy for Healthcare of Indigenous Peoples (Pnaspi) in the Alto Rio Negro Amazon region was analyzed based on the principles of the differentiated care model. This theme was investigated from three perspectives, namely the formulation of the guidelines, the therapeutic itineraries in indigenous villages, and the work routines of the Indigenous Community Health Agents (AIS). It involved qualitative research based on the anthropological perspective of Eduardo Menéndez. The techniques used were participant observation, interviews, and, document analysis. The formulation of the guidelines of differentiated care emphasizes the adaptation of technologies and professionals, minimizing the coexistence and disputes among healthcare models. Menéndez's perspective focused on the viewpoint of the subjects was crucial for identifying the coexistence and articulation among different forms of care in the villages through the healthcare itineraries. Nevertheless, it was not possible to identify the inclusion of indigenous health practices and representations through the work routines of the Indigenous Community Health Agents (AIS). The focus was on self-care developed and practiced by the subjects and their families.