Surface-enhanced resonance Raman scattering (SERRS) simulates PCR for sensitive DNA detection

Haibo Zhou; Shenyu Lin; Yichu Nie; Danting Yang; Qiqin Wang; Weijia Chen; Ning Huang; Zhengjin Jiang; Shanze Chen

doi:10.1039/c5an01887k

Surface-enhanced resonance Raman scattering (SERRS) simulates PCR for sensitive DNA detection

Analyst. 2015 Nov 21;140(22):7518-21. doi: 10.1039/c5an01887k.

Authors

Haibo Zhou¹, Shenyu Lin², Yichu Nie³, Danting Yang⁴, Qiqin Wang¹, Weijia Chen¹, Ning Huang⁵, Zhengjin Jiang¹, Shanze Chen⁵

Affiliations

¹ Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou, Guangdong Province 510632, China. jzjjackson@hotmail.com.
² Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou, Guangdong Province 510632, China. jzjjackson@hotmail.com and Department of Hand Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province 430022, China.
³ State Key Laboratory of Respiratory Diseases, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, No. 151 Yanjiang Road, Yuexiu Dist., Guangzhou, Guangdong Province 510120, China.
⁴ Schools of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang Province 310058, China.
⁵ Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan Province 610041, China.

PMID: 26465756
DOI: 10.1039/c5an01887k

Abstract

This paper describes a novel double-stranded DNA detection method through resonance between SYBR Green I and DNA with the surface-enhanced resonance Raman scattering (SERRS) assay, which opens an avenue to the quantitative and reliable application of SERRS in DNA detection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Benzothiazoles
DNA / analysis*
Diamines
Fluorescent Dyes / chemistry*
Organic Chemicals / chemistry*
Polymerase Chain Reaction / methods*
Quinolines
Spectrum Analysis, Raman / methods*

Substances

Benzothiazoles
Diamines
Fluorescent Dyes
Organic Chemicals
Quinolines
SYBR Green I
DNA