Sensitive discrimination of stable mismatched base pairs by an abasic site modified fluorescent probe and lambda exonuclease

Tongbo Wu; Xianjin Xiao; Feidan Gu; Meiping Zhao

doi:10.1039/c5cc05749c

Sensitive discrimination of stable mismatched base pairs by an abasic site modified fluorescent probe and lambda exonuclease

Chem Commun (Camb). 2015 Dec 21;51(98):17402-5. doi: 10.1039/c5cc05749c.

Authors

Tongbo Wu¹, Xianjin Xiao¹, Feidan Gu¹, Meiping Zhao¹

Affiliation

¹ Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China. mpzhao@pku.edu.cn.

PMID: 26465212
DOI: 10.1039/c5cc05749c

Abstract

An abasic site modified fluorescent probe has been developed which enabled the rapid discrimination of stable single mismatched base pairs by lambda exonuclease with remarkably high discrimination factors (447 for T:G and 238 for A:G). This method allowed sensitive detection of single nucleotide variation at very low abundances (0.02-0.05% mutant-to-wild type).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Pair Mismatch*
Base Sequence
DNA Probes / chemistry
DNA Probes / genetics*
Exodeoxyribonucleases / metabolism*
Fluorescent Dyes / chemistry*
Furans / chemistry
Janus Kinase 2 / genetics
Molecular Probe Techniques
Point Mutation
Uracil-DNA Glycosidase / genetics

Substances

DNA Probes
Fluorescent Dyes
Furans
Janus Kinase 2
Exodeoxyribonucleases
Uracil-DNA Glycosidase