Biosensor for label-free DNA quantification based on functionalized LPGs

Biosens Bioelectron. 2016 Oct 15:84:30-6. doi: 10.1016/j.bios.2015.10.001. Epub 2015 Oct 3.

Abstract

A label-free fiber optic biosensor based on a long period grating (LPG) and a basic optical interrogation scheme using off the shelf components is used for the detection of in-situ DNA hybridization. A new methodology is proposed for the determination of the spectral position of the LPG mode resonance. The experimental limit of detection obtained for the DNA was 62±2nM and the limit of quantification was 209±7nM. The sample specificity was experimentally demonstrated using DNA targets with different base mismatches relatively to the probe and was found that the system has a single base mismatch selectivity.

Keywords: Biosensing; DNA; Detection; Label-free; Quantification.

Publication types

  • Evaluation Study

MeSH terms

  • Base Pair Mismatch
  • Biosensing Techniques / instrumentation*
  • Biosensing Techniques / methods
  • DNA / analysis*
  • DNA / genetics
  • Equipment Design
  • Fiber Optic Technology / instrumentation*
  • Fiber Optic Technology / methods
  • In Situ Hybridization / instrumentation*
  • In Situ Hybridization / methods
  • Limit of Detection
  • Models, Molecular

Substances

  • DNA