Bright fluorogenic squaraines with tuned cell entry for selective imaging of plasma membrane vs. endoplasmic reticulum

Chem Commun (Camb). 2015 Dec 14;51(96):17136-9. doi: 10.1039/c5cc06094j.

Abstract

A rational design of squaraine dyes with lipophilic and zwitterionic groups tunes cell entry, allowing for selective far-red/near-infrared imaging of plasma membrane vs. endoplasmic reticulum. They exhibit up to 110-fold fluorescence enhancement in biomembranes and enable cellular imaging at 1 nM concentration, which make them the brightest membrane probes to date.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Membrane / chemistry*
  • Cyclobutanes / chemical synthesis
  • Cyclobutanes / chemistry*
  • Endoplasmic Reticulum / chemistry*
  • Fluorescence
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • HeLa Cells
  • Humans
  • Molecular Structure
  • Phenols / chemical synthesis
  • Phenols / chemistry*

Substances

  • Cyclobutanes
  • Fluorescent Dyes
  • Phenols
  • squaraine