Characterization of inner membrane protein YciB in Escherichia coli: YciB interacts with cell elongation and division proteins

Abstract

The function of inner membrane protein YciB in Escherichia coli has not been identified. In this study, the membrane topology of the protein that contains five transmembrane domains was clarified. YciB was found to interact with various proteins involved in cell elongation and cell division using a bacterial two-hybrid system. It was also found that the deletion mutant of yciB is susceptible to the low osmolarity. These observations together with previous reports indicate that YciB is involved in synthesis of the cell envelope by interacting with cell elongation and cell division complexes.

Keywords: Escherichia coli; YciB; membrane topology; two-hybrid analysis.