Liposomally formulated phospholipid-conjugated indocyanine green for intra-operative brain tumor detection and resection

Akiko Suganami; Yasuo Iwadate; Sayaka Shibata; Masamichi Yamashita; Tsutomu Tanaka; Natsuki Shinozaki; Ichio Aoki; Naokatsu Saeki; Hiroshi Shirasawa; Yoshiharu Okamoto; Yutaka Tamura

doi:10.1016/j.ijpharm.2015.10.001

Liposomally formulated phospholipid-conjugated indocyanine green for intra-operative brain tumor detection and resection

Int J Pharm. 2015 Dec 30;496(2):401-6. doi: 10.1016/j.ijpharm.2015.10.001. Epub 2015 Oct 23.

Authors

Affiliations

¹ Department of Bioinformatics, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan; Department of Molecular Virology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan; Molecular Chirality Research Center, Chiba University, Chiba 263-8522, Japan.
² Department of Neurological Surgery, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.
³ Department of Bioinformatics, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan; Molecular Imaging Center, National Institute of Radiological Sciences, Chiba 263-8555, Japan.
⁴ Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
⁵ Molecular Imaging Center, National Institute of Radiological Sciences, Chiba 263-8555, Japan.
⁶ Department of Molecular Virology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.
⁷ Department of Bioinformatics, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan; Molecular Chirality Research Center, Chiba University, Chiba 263-8522, Japan. Electronic address: yutaka_tamura@faculty.chiba-u.jp.

PMID: 26453781
DOI: 10.1016/j.ijpharm.2015.10.001

Abstract

Some tumor-specific near-infrared (NIR) fluorescent dyes such as indocyanine green (ICG), IDRye800CW, and 5-aminolevulinic acid have been used clinically for detecting tumor margins or micro-cancer lesions. In this study, we evaluated the physicochemical properties of liposomally formulated phospholipid-conjugated ICG, denoted by LP-iDOPE, as a clinically translatable NIR imaging nanoparticle for brain tumors. We also confirmed its brain-tumor-specific biodistribution and its characteristics as the intra-operative NIR imaging nanoparticles for brain tumor surgery. These properties of LP-iDOPE may enable neurosurgeons to achieve more accurate identification and more complete resection of brain tumor.

Keywords: Brain tumor surgery; Indocyanine green (ICG); Intra-operative imaging; Liposome; Near-infrared (NIR).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain Neoplasms / diagnosis*
Brain Neoplasms / surgery
Chemistry, Pharmaceutical
Indocyanine Green / administration & dosage*
Liposomes / chemistry
Male
Phosphatidylcholines / chemistry
Phosphatidylethanolamines / chemistry
Phospholipids / chemistry*
Rats
Rats, Inbred F344
Spectrometry, Fluorescence
Spectroscopy, Near-Infrared

Substances

Liposomes
Phosphatidylcholines
Phosphatidylethanolamines
Phospholipids
dioleoyl phosphatidylethanolamine
1,2-oleoylphosphatidylcholine
Indocyanine Green