Study of exhaled breath condensate sample preparation for metabolomics analysis by LC-MS/MS in high resolution mode

M A Fernández-Peralbo; M Calderón Santiago; F Priego-Capote; M D Luque de Castro

doi:10.1016/j.talanta.2015.08.010

Study of exhaled breath condensate sample preparation for metabolomics analysis by LC-MS/MS in high resolution mode

Talanta. 2015 Nov 1:144:1360-9. doi: 10.1016/j.talanta.2015.08.010. Epub 2015 Aug 6.

Authors

M A Fernández-Peralbo¹, M Calderón Santiago¹, F Priego-Capote², M D Luque de Castro³

Affiliations

¹ Department of Analytical Chemistry, Annex Marie Curie Building, Campus of Rabanales, University of Córdoba, E-14071 Córdoba, Spain; Institute of Biomedical Research Maimónides (IMIBIC), Reina Sofía Hospital, University of Córdoba, E-14004 Córdoba, Spain.
² Department of Analytical Chemistry, Annex Marie Curie Building, Campus of Rabanales, University of Córdoba, E-14071 Córdoba, Spain; Institute of Biomedical Research Maimónides (IMIBIC), Reina Sofía Hospital, University of Córdoba, E-14004 Córdoba, Spain. Electronic address: q72prcaf@uco.es.
³ Department of Analytical Chemistry, Annex Marie Curie Building, Campus of Rabanales, University of Córdoba, E-14071 Córdoba, Spain; Institute of Biomedical Research Maimónides (IMIBIC), Reina Sofía Hospital, University of Córdoba, E-14004 Córdoba, Spain. Electronic address: qa1lucam@uco.es.

PMID: 26452970
DOI: 10.1016/j.talanta.2015.08.010

Abstract

Metabolomic analysis of exhaled breath condensate (EBC) requires an unavoidable sample preparation step because of the low concentration of its components, and potential cleanup for possible interferents. Sample preparation based on protein precipitation (PP), solid-phase extraction (SPE) by hydrophilic and lipophilic sorbents or lyophilization has demonstrated that the analytical sample from the last is largely the best because lyophilization allows reconstitution in a volume as small as required (preconcentration factors up to 80-times with respect to the original sample), thus doubling the number of detected compounds as compared with the other alternatives (47 versus 25). In addition, PP and/or SPE cleanup are unnecessary as no effect from the EBC components removed by these steps appears in the chromatograms. The total 49 EBC compounds tentatively identified and confirmed by MS/MS in this research include amino acids, fatty acids, fatty amides, fatty aldehydes, sphingoid bases, oxoanionic compounds, imidazoles, hydroxy acids and aliphatic acyclic acids.

Keywords: Exhaled breath condensate; LC–QTOF; Lyophilization; Metabolomics; Sample preparation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Analytic Sample Preparation Methods / methods*
Breath Tests / methods*
Chromatography, Liquid / methods*
Exhalation*
Freeze Drying
Humans
Hydrophobic and Hydrophilic Interactions
Metabolomics / methods*
Solid Phase Extraction
Tandem Mass Spectrometry / methods*