The fluorescence detection of glutathione by ∙OH radicals' elimination with catalyst of MoS2/rGO under full spectrum visible light irradiation

Nan Zhang; Weiguang Ma; Dongxue Han; Lingnan Wang; Tongshun Wu; Li Niu

doi:10.1016/j.talanta.2015.07.003

The fluorescence detection of glutathione by ∙OH radicals' elimination with catalyst of MoS2/rGO under full spectrum visible light irradiation

Talanta. 2015 Nov 1:144:551-8. doi: 10.1016/j.talanta.2015.07.003. Epub 2015 Jul 5.

Authors

Nan Zhang¹, Weiguang Ma¹, Dongxue Han², Lingnan Wang¹, Tongshun Wu³, Li Niu²

Affiliations

¹ State Key Laboratory of Electroanalytical Chemistry, c/o Engineering Laboratory for Modern Analytical Techniques, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China; University of the Chinese Academy of Sciences, Beijing 100039, China.
² State Key Laboratory of Electroanalytical Chemistry, c/o Engineering Laboratory for Modern Analytical Techniques, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China.
³ State Key Laboratory of Electroanalytical Chemistry, c/o Engineering Laboratory for Modern Analytical Techniques, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China. Electronic address: tswu@ciac.ac.cn.

PMID: 26452861
DOI: 10.1016/j.talanta.2015.07.003

Abstract

In this study, a new method for the detection of glutathione (GSH) was designed based on the ∙OH radicals' elimination system due to the reducing ability of GSH for the first time. Fluorescence method with terephthalic acid (TA) as the probe was employed for the quantification of ∙OH radicals' production and elimination. Experimental conditions of ∙OH radicals' production were optimized in detail, and ∙OH radicals were found to be efficiently produced by the excellent catalysis performance of MoS2/rGO under full spectrum visible light irradiation. The introduction of GSH make fluorescent intensity decrease due to the elimination of ∙OH radicals. For the present fluorescence based GSH sensor, a wide detection range of 60.0-700.0 µM and excellent selectivity have been achieved. Furthermore, it has been successfully employed for the determination of GSH in commercial drug tablets and human serum.

Keywords: Fluorescent assay; Glutathione; MoS(2)/rGO; ∙OH radical.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Catalysis
Disulfides / chemistry*
Disulfides / radiation effects
Fluorescence
Glutathione / analysis*
Glutathione / blood
Glutathione / chemistry
Graphite / chemistry*
Graphite / radiation effects
Humans
Hydroxyl Radical / chemistry*
Light
Molybdenum / chemistry*
Molybdenum / radiation effects
Oxidation-Reduction
Oxides / chemistry*
Oxides / radiation effects
Phthalic Acids / chemistry
Tablets / chemistry

Substances

Disulfides
Oxides
Phthalic Acids
Tablets
Hydroxyl Radical
terephthalic acid
Graphite
Molybdenum
Glutathione
molybdenum disulfide