Combination of biological pretreatment with NaOH/Urea pretreatment at cold temperature to enhance enzymatic hydrolysis of rice straw

Youzhi Dai; Mengying Si; Yuehui Chen; Nianlei Zhang; Mo Zhou; Qi Liao; Deqiang Shi; Yine Liu

doi:10.1016/j.biortech.2015.09.091

Combination of biological pretreatment with NaOH/Urea pretreatment at cold temperature to enhance enzymatic hydrolysis of rice straw

Bioresour Technol. 2015 Dec:198:725-31. doi: 10.1016/j.biortech.2015.09.091. Epub 2015 Oct 3.

Authors

Youzhi Dai¹, Mengying Si¹, Yuehui Chen², Nianlei Zhang¹, Mo Zhou¹, Qi Liao³, Deqiang Shi⁴, Yine Liu¹

Affiliations

¹ Department of Environmental Science and Engineering, Xiangtan University, Xiangtan City 411105, China.
² Department of Environmental Science and Engineering, Xiangtan University, Xiangtan City 411105, China. Electronic address: 61080510@qq.com.
³ Department of Environmental Engineering, School of Metallurgy and Environment, Central South University, Changsha 410083, China; Chinese National Engineering Research Centre for Control and Treatment of Heavy Metal Pollution, Changsha 410083, China.
⁴ Guangxi Academy of Sciences, Nanning City, Guangxi Zhuang Autonomous Region 530007, China.

PMID: 26452179
DOI: 10.1016/j.biortech.2015.09.091

Abstract

A stepwise pretreatment of combination of bacterial treatment with NaOH/Urea (NU) treatment was conducted to enhance enzymatic hydrolysis of rice straw (RS). The results showed that the composition of RS changed significantly, the lignin and hemicellulose decreased while cellulose increased. The biological treatment with a bacterium Sphingobacterium sp. LD-1 achieved mild conditions for the sequential NU treatment, reducing the concentration of the NU solution from 7%/12% to 4%/6% and increasing the temperature from -20°C to -10°C. The saccharification of rice straw co-treated with bacterium Sphingobacterium sp. LD-1 and 4%/6% NU at -10°C resulted in 1.396-fold and 1.372-fold increase of reducing sugar and glucose yield respectively than that of sole NU treatment.

Keywords: Enzymatic hydrolysis; Lignocellulose pretreatment; NaOH/Urea; Rice straw; Sphingobacterium sp. LD-1.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Biofuels*
Carbohydrates
Cellulose / metabolism
Cold Temperature
Hydrolysis
Lignin / metabolism
Oryza / metabolism*
Polysaccharides / metabolism
Sodium Hydroxide*
Sphingobacterium / metabolism*
Urea*

Substances

Biofuels
Carbohydrates
Polysaccharides
Sodium Hydroxide
hemicellulose
Urea
Cellulose
Lignin