Complete Genome Sequence of the Clostridium difficile Type Strain DSM 1296T

Thomas Riedel; Boyke Bunk; Johannes Wittmann; Andrea Thürmer; Cathrin Spröer; Sabine Gronow; Heiko Liesegang; Rolf Daniel; Jörg Overmann

doi:10.1128/genomeA.01186-15

Complete Genome Sequence of the Clostridium difficile Type Strain DSM 1296T

Genome Announc. 2015 Oct 8;3(5):e01186-15. doi: 10.1128/genomeA.01186-15.

Authors

Thomas Riedel¹, Boyke Bunk², Johannes Wittmann³, Andrea Thürmer⁴, Cathrin Spröer², Sabine Gronow², Heiko Liesegang⁴, Rolf Daniel⁴, Jörg Overmann²

Affiliations

¹ Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany thomas.riedel@dsmz.de.
² Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany German Centre of Infection Research (DZIF), Partner site Hannover-Braunschweig, Braunschweig, Germany.
³ Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
⁴ Department of Genomics and Applied Microbiology and Göttingen Genomics Laboratory, Georg-August-University, Göttingen, Germany.

Abstract

In this study, we sequenced the complete genome of the Clostridium difficile type strain DSM 1296(T). A combination of single-molecule real-time (SMRT) and Illumina sequencing technology revealed the presence of one chromosome and two extrachromosomal elements, the bacteriophage phiCDIF1296T and a putative plasmid-like structure harboring genes of another bacteriophage.