Nanostructured Capsules for Cartilage Tissue Engineering

Methods Mol Biol. 2015:1340:181-9. doi: 10.1007/978-1-4939-2938-2_13.

Abstract

Polymeric multilayered capsules (PMCs) have found great applicability in bioencapsulation, an evolving branch of tissue engineering and regenerative medicine. Here, we describe the production of hierarchical PMCs composed by an external multilayered membrane by layer-by-layer assembly of poly(L-lysine), alginate, and chitosan. The core of the PMCs is liquified and encapsulates human adipose stem cells and surface-functionalized collagen II-TGF-β3 poly(L-lactic acid) microparticles for cartilage tissue engineering.

Keywords: Bioencapsulation; Capsules; Cartilage regeneration; Collagen II; Layer-by-layer; Microparticles; Stem cells; TGF-β3; Tissue engineering.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alginates / chemistry
  • Cartilage / cytology*
  • Cartilage / drug effects
  • Cartilage / metabolism
  • Cartilage / transplantation
  • Cell Culture Techniques
  • Cell Differentiation
  • Cells, Cultured
  • Chitosan / chemistry
  • Chondrocytes / drug effects
  • Chondrocytes / metabolism
  • Chondrocytes / physiology*
  • Chondrocytes / transplantation
  • Chondrogenesis* / drug effects
  • Collagen Type II / metabolism
  • Glucuronic Acid / chemistry
  • Hexuronic Acids / chemistry
  • Humans
  • Nanocapsules*
  • Nanomedicine / methods*
  • Polylysine / chemistry
  • Polymers / chemistry*
  • Regeneration
  • Regenerative Medicine / methods*
  • Stem Cell Transplantation
  • Stem Cells / drug effects
  • Stem Cells / metabolism
  • Stem Cells / physiology*
  • Subcutaneous Fat / cytology
  • Tissue Engineering / methods*
  • Tissue Scaffolds*
  • Transforming Growth Factor beta3 / administration & dosage
  • Transforming Growth Factor beta3 / metabolism

Substances

  • Alginates
  • Collagen Type II
  • Hexuronic Acids
  • Nanocapsules
  • Polymers
  • Transforming Growth Factor beta3
  • Polylysine
  • Glucuronic Acid
  • Chitosan