Dietary NiCl₂ causes G₂/M cell cycle arrest in the broiler's kidney

Hongrui Guo; Hengmin Cui; Xi Peng; Jing Fang; Zhicai Zuo; Junliang Deng; Xun Wang; Bangyuan Wu; Kejie Chen; Jie Deng

doi:10.18632/oncotarget.5934

Dietary NiCl₂ causes G₂/M cell cycle arrest in the broiler's kidney

Oncotarget. 2015 Nov 3;6(34):35964-77. doi: 10.18632/oncotarget.5934.

Authors

Hongrui Guo¹, Hengmin Cui^{1

2}, Xi Peng^{1

2}, Jing Fang^{1

2}, Zhicai Zuo^{1

2}, Junliang Deng^{1

2}, Xun Wang^{1

2}, Bangyuan Wu¹, Kejie Chen¹, Jie Deng¹

Affiliations

¹ Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Ya'an, China.
² College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, China.

Abstract

Here we showed that dietary NiCl2 in excess of 300 mg/kg caused the G2/M cell cycle arrest and the reduction of cell proportion at S phase. The G2/M cell cycle arrest was accompanied by up-regulation of phosphorylated ataxia telangiectasia mutated (p-ATM), p53, p-Chk1, p-Chk2, p21 protein expression and ATM, p53, p21, Chk1, Chk2 mRNA expression, and down-regulation of p-cdc25C, cdc2, cyclinB and proliferating cell nuclear antigen (PCNA) protein expression and the cdc25, cdc2, cyclinB, PCNA mRNA expression.

Keywords: G2/M cell cycle arrest; NiCl2; kidney; mRNA expression; protein expression.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Ataxia Telangiectasia Mutated Proteins / genetics
Ataxia Telangiectasia Mutated Proteins / metabolism
Chickens
G2 Phase Cell Cycle Checkpoints / drug effects*
Kidney / cytology*
Kidney / drug effects*
Nickel / administration & dosage*
Phosphorylation
Protein Kinases / metabolism
Signal Transduction
Tumor Suppressor Proteins / metabolism

Substances

Tumor Suppressor Proteins
nickel chloride
Nickel
Protein Kinases
Ataxia Telangiectasia Mutated Proteins