Autoantibodies against vinculin in patients with chronic inflammatory demyelinating polyneuropathy

Minako Beppu; Setsu Sawai; Mamoru Satoh; Masahiro Mori; Takahiro Kazami; Sonoko Misawa; Kazumoto Shibuya; Masumi Ishibashi; Kazuyuki Sogawa; Sayaka Kado; Yoshio Kodera; Fumio Nomura; Satoshi Kuwabara

doi:10.1016/j.jneuroim.2015.07.012

Autoantibodies against vinculin in patients with chronic inflammatory demyelinating polyneuropathy

J Neuroimmunol. 2015 Oct 15:287:9-15. doi: 10.1016/j.jneuroim.2015.07.012. Epub 2015 Jul 29.

Authors

Affiliations

¹ Department of Neurology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan; Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan. Electronic address: beppu@chiba-u.jp.
² Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
³ Department of Neurology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
⁴ Chemical Analysis Center, Chiba University, 1-33 Yayoicho, Inage-ku, Chiba 263-8522, Japan.
⁵ Department of Physics, School of Science, Kitasato University, 1-15-1, Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0373, Japan.

PMID: 26439954
DOI: 10.1016/j.jneuroim.2015.07.012

Abstract

To identify the target molecules of chronic inflammatory demyelinating polyneuropathy (CIDP), we used proteomic-based approach in the extracted proteins from porcine cauda equina. Two of 31 CIDP patients had markedly elevated serum autoantibodies against vinculin, a cell adhesion protein. Both of the patients with anti-vinculin antibodies had similar clinical manifestation, which are compatible with those of "typical" CIDP. Immunocytochemistry showed that vinculin was stained at the myelin sheath of the sciatic nerves by serum samples. Our results suggest that vinculin is a possible immunological target molecule in a subpopulation of typical CIDP patients.

Keywords: Autoantibody; Chronic inflammatory demyelinating polyneuropathy; Epitope; Vinculin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Autoantibodies / blood*
Case-Control Studies
Female
Humans
Male
Multiple Sclerosis / blood
Multiple Sclerosis / immunology
Myasthenia Gravis / blood
Myasthenia Gravis / immunology
Myelin Sheath / metabolism
Neuromyelitis Optica / blood
Neuromyelitis Optica / immunology
Polyradiculoneuropathy, Chronic Inflammatory Demyelinating / blood*
Polyradiculoneuropathy, Chronic Inflammatory Demyelinating / immunology*
Polyradiculoneuropathy, Chronic Inflammatory Demyelinating / pathology
Proteomics
Schwann Cells / metabolism
Swine
Vinculin / immunology*

Substances

Autoantibodies
Vinculin