Klebsiella sp. FIRD 2, a TBT-resistant bacterium isolated from contaminated surface sediment along Strait of Johor Malaysia

Abdussamad Abubakar; Muskhazli B Mustafa; Wan Lutfi Wan Johari; Syaizwan Zahmir Zulkifli; Ahmad Ismail; Ferdaus Binti Mohamat-Yusuff

doi:10.1016/j.marpolbul.2015.09.041

Klebsiella sp. FIRD 2, a TBT-resistant bacterium isolated from contaminated surface sediment along Strait of Johor Malaysia

Mar Pollut Bull. 2015 Dec 15;101(1):280-283. doi: 10.1016/j.marpolbul.2015.09.041. Epub 2015 Oct 3.

Authors

Abdussamad Abubakar¹, Muskhazli B Mustafa², Wan Lutfi Wan Johari³, Syaizwan Zahmir Zulkifli², Ahmad Ismail², Ferdaus Binti Mohamat-Yusuff⁴

Affiliations

¹ Department of Environmental Sciences, Faculty of Environmental Studies, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia; Environmental Forensics Research Centre ENFORCE, Fakulti of Environmental Studies, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Bauchi State University Gadau, PMB 65, Itas Gadau Bauchi, Nigeria.
² Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia.
³ Department of Environmental Sciences, Faculty of Environmental Studies, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia; Environmental Forensics Research Centre ENFORCE, Fakulti of Environmental Studies, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
⁴ Department of Environmental Sciences, Faculty of Environmental Studies, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia; Environmental Forensics Research Centre ENFORCE, Fakulti of Environmental Studies, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. Electronic address: ferdius@upm.edu.my.

PMID: 26434791
DOI: 10.1016/j.marpolbul.2015.09.041

Abstract

A possible tributyltin (TBT)-degrading bacterium isolated from contaminated surface sediment was successfully identified as Klebsiella sp. FIRD 2. It was found to be the best isolate capable of resisting TBT at a concentration of 1000 μg L(-1). This was a concentration above the reported contaminated level at the sampling station, 790 μg L(-1). Further studies revealed that the isolate was Gram negative and resisted TBT concentrations of up to 1500 μg L(-1) in a Minimal Salt Broth without the addition of any carbon source within the first 48 h of incubation. It is expected that additional work could be conducted to check the degradation activity of this new isolate and possibly improve the degradation capacity in order to contribute to finding a safe and sustainable remediation solution of TBT contamination.

Keywords: Degradation; Malaysia; Microorganism; Organotin; Pollution; TBT-resistant.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biodegradation, Environmental
Drug Resistance, Bacterial*
Geologic Sediments / microbiology*
Klebsiella / drug effects
Klebsiella / isolation & purification*
Malaysia
Trialkyltin Compounds / analysis*
Water Pollutants, Chemical / analysis*

Substances

Trialkyltin Compounds
Water Pollutants, Chemical
tributyltin