The goal of this study was the investigation of the relationship between 200 m performance and motor abilities and anthropometric characteristics of different level of male sprinters (200 m performance 23.80 ± 2.16 s--the best results 21.40 s). The physical fitness measures included: 50 m from standing and flying start, standing long jump (SLJ) standing five jump (SFJ), double and single leg countermovement jumps CMJ), flexibility (sit and reach) and 4 kg shot put throwing (over head). The Spearman correlation coefficient was applied to verify the association. The results demonstrated strong relationships (p < 0.05) between 200 m performance and experience (age) and body mass (r = 0.85, r = -0.80 respectively) As for the motor abilities strong relationship exists between 200 m and time of 150 m, 50 m from standing and flying start and CMJ on single leg. Vertical jumping displayed stronger relationship with 200 m performance that horizontal one. From a practical point of view this is very important notice.