Construction and immunological evaluation of recombinant Lactobacillus plantarum expressing HN of Newcastle disease virus and DC- targeting peptide fusion protein

Yanlong Jiang; Jingtao Hu; Yanbing Guo; Wentao Yang; Liping Ye; Chunwei Shi; Yuying Liu; Guilian Yang; Chunfeng Wang

doi:10.1016/j.jbiotec.2015.09.033

Construction and immunological evaluation of recombinant Lactobacillus plantarum expressing HN of Newcastle disease virus and DC- targeting peptide fusion protein

J Biotechnol. 2015 Dec 20:216:82-9. doi: 10.1016/j.jbiotec.2015.09.033. Epub 2015 Sep 30.

Authors

Yanlong Jiang¹, Jingtao Hu¹, Yanbing Guo¹, Wentao Yang¹, Liping Ye¹, Chunwei Shi¹, Yuying Liu¹, Guilian Yang², Chunfeng Wang³

Affiliations

¹ College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China.
² College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China. Electronic address: yangguilian@jlau.edu.cn.
³ College of Animal Science and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China. Electronic address: wangchunfeng@jlau.edu.cn.

PMID: 26432338
DOI: 10.1016/j.jbiotec.2015.09.033

Abstract

Newcastle disease virus (NDV) has been considered as one of the most severe threats to poultry industry. In this study, we constructed a series of food grade recombinant Lactobacillus plantarum (L. plantarum) strains (RLP) synthesizing either virus hemagglutinin-neuraminidase protein (HN) alone or HN fused with DC cells targeting peptides (DCpep), named RLP(pSIP409-HN) and RLP (pSIP409-HN-DCpep), respectively. The immune responses and protective efficacy were then evaluated in chickens. Results showed that the presence of DCpep in RLP (pSIP409-HN-DCpep) group significantly increased the production of secretory immunoglobulin A (SIgA) in intestines and the percentages of CD3(+)CD4(+) T cells in spleen and peripheral blood leukocytes (P<0.05) compared to chickens immunized with RLP (pSIP409-HN). In addition, the similar enhancement effects were also observed with regard to trachea SIgA, T lymphocytes proliferation and survival rates after NDV challenge, even without significant statics differences. The results demonstrated the possibility to take use of DCpep as an immune adjuvant in the design of NDV vaccine.

Keywords: DC-targeting peptide; HN protein; Immune response; L. plantarum; NDV.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Viral / immunology
Antibody Formation / immunology
Cell Proliferation
Chickens
Dendritic Cells / immunology*
HN Protein / immunology*
Hemagglutination Inhibition Tests
Lactobacillus plantarum / immunology*
Newcastle disease virus / immunology*
Newcastle disease virus / pathogenicity
Peptides / immunology*
Recombinant Fusion Proteins / immunology*
T-Lymphocytes / immunology
Virulence

Substances

Antibodies, Viral
HN Protein
Peptides
Recombinant Fusion Proteins