Massilia timonae as cause of chronic endophthalmitis following cataract surgery

J Cataract Refract Surg. 2015 Aug;41(8):1778-80. doi: 10.1016/j.jcrs.2015.07.016.

Abstract

This is the first report of chronic postoperative endophthalmitis caused by Massilia timonae. The gram-negative bacillus was isolated from a patient who developed chronic endophthalmitis after cataract extraction. The microorganism was cultured on brain-heart infusion broth and identified using the 16S rRNA sequence analysis. Biochemically, the organism was positive for catalase and oxidase and negative for arginine dihydrolase. It was pansensitive to β-lactams, fluoroquinolones, aminoglycosides, and trimethoprim-sulfamethoxazole but resistant to aztreonam. Massilia timonae is a new bacterium implicated in postoperative chronic endophthalmitis. Eubacterial polymerase chain reaction was useful in identifying M timonae because phenotyping testing and conventional algorithms could not identify it.

Financial disclosure: No author has a financial or proprietary interest in any material or method mentioned.

Publication types

  • Case Reports

MeSH terms

  • Aged
  • Anti-Bacterial Agents / therapeutic use
  • Ceftazidime / therapeutic use
  • Chronic Disease
  • DNA, Bacterial / analysis
  • Drug Therapy, Combination
  • Endophthalmitis / diagnosis
  • Endophthalmitis / drug therapy
  • Endophthalmitis / microbiology*
  • Eye Infections, Bacterial / diagnosis
  • Eye Infections, Bacterial / drug therapy
  • Eye Infections, Bacterial / microbiology*
  • Female
  • Gram-Negative Bacterial Infections / diagnosis
  • Gram-Negative Bacterial Infections / drug therapy
  • Gram-Negative Bacterial Infections / microbiology*
  • Humans
  • Intravitreal Injections
  • Lens Implantation, Intraocular
  • Microbial Sensitivity Tests
  • Oxalobacteraceae / genetics
  • Oxalobacteraceae / isolation & purification*
  • Phacoemulsification*
  • Polymerase Chain Reaction
  • Postoperative Complications*
  • RNA, Ribosomal, 16S / genetics
  • Tobramycin / therapeutic use
  • Vancomycin / therapeutic use

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • RNA, Ribosomal, 16S
  • Vancomycin
  • Ceftazidime
  • Tobramycin