Complete Genome Sequence of Noncytopathic Bovine Viral Diarrhea Virus 1 Contaminating a High-Passage RK-13 Cell Line

Bora Nam; Ganwu Li; Ying Zheng; Jianqiang Zhang; Kathleen M Shuck; Peter J Timoney; Udeni B R Balasuriya

doi:10.1128/genomeA.01115-15

Complete Genome Sequence of Noncytopathic Bovine Viral Diarrhea Virus 1 Contaminating a High-Passage RK-13 Cell Line

Genome Announc. 2015 Oct 1;3(5):e01115-15. doi: 10.1128/genomeA.01115-15.

Authors

Bora Nam¹, Ganwu Li², Ying Zheng², Jianqiang Zhang², Kathleen M Shuck¹, Peter J Timoney¹, Udeni B R Balasuriya³

Affiliations

¹ Department of Veterinary Science, Maxwell H. Gluck Equine Research Center, University of Kentucky, Lexington, Kentucky, USA.
² Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA.
³ Department of Veterinary Science, Maxwell H. Gluck Equine Research Center, University of Kentucky, Lexington, Kentucky, USA ubalasuriya@uky.edu.

Abstract

A high-passage rabbit kidney RK-13 cell line (HP-RK-13[KY], originally derived from the ATCC CCL-37 cell line) used in certain laboratories worldwide is contaminated with noncytopathic bovine viral diarrhea virus (ncpBVDV). On complete genome sequence analysis, the virus strain was found to belong to BVDV group 1b.