Yeast Mitochondria as a Model System to Study the Biogenesis of Bacterial β-Barrel Proteins

Methods Mol Biol. 2015:1329:17-31. doi: 10.1007/978-1-4939-2871-2_2.

Abstract

Beta-barrel proteins are found in the outer membrane of Gram-negative bacteria, mitochondria, and chloroplasts. The evolutionary conservation in the biogenesis of these proteins allows mitochondria to assemble bacterial β-barrel proteins in their functional form. In this chapter, we describe exemplarily how the capacity of yeast mitochondria to process the trimeric autotransporter YadA can be used to study the role of bacterial periplasmic chaperones in this process.

Keywords: BAM complex; Chaperones; Evolutionary conservation; Mitochondria; Skp; TOB complex; β-Barrel proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adhesins, Bacterial / biosynthesis*
  • Adhesins, Bacterial / chemistry
  • Adhesins, Bacterial / genetics
  • Adhesins, Bacterial / metabolism
  • Blotting, Western
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Interleukin-8 / metabolism
  • Mitochondria / metabolism*
  • Protein Structure, Secondary
  • Saccharomyces cerevisiae / cytology*
  • Saccharomyces cerevisiae / genetics*
  • Transformation, Genetic

Substances

  • Adhesins, Bacterial
  • Interleukin-8
  • YadA protein, Yersinia