Micro-optical coherence tomography tracking of magnetic gene transfection via Au-Fe3O4 dumbbell nanoparticles

Wei Shi; Xinyu Liu; Chao Wei; Zhichuan J Xu; Stanley Siong Wei Sim; Linbo Liu; Chenjie Xu

doi:10.1039/c5nr05459a

Micro-optical coherence tomography tracking of magnetic gene transfection via Au-Fe3O4 dumbbell nanoparticles

Nanoscale. 2015 Nov 7;7(41):17249-53. doi: 10.1039/c5nr05459a.

Authors

Wei Shi¹, Xinyu Liu, Chao Wei, Zhichuan J Xu, Stanley Siong Wei Sim, Linbo Liu, Chenjie Xu

Affiliation

¹ School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, Singapore 637457.

PMID: 26425826
DOI: 10.1039/c5nr05459a

Abstract

Heterogeneous Au-Fe3O4 dumbbell nanoparticles (NPs) are composed of Au NPs and Fe3O4 NPs that bring in optical and magnetic properties respectively. This article reports the engineering of Au-Fe3O4 NPs as gene carriers for magnetic gene transfection as well as contrast agents for micro-optical coherence tomography (μOCT). As a proof-of-concept, Au-Fe3O4 NPs are used to deliver the green fluorescent protein to HEK 293T cells and their entrance into the cells is monitored through μOCT.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ferric Compounds / chemistry*
Gold / chemistry*
HEK293 Cells
Humans
Nanoparticles / chemistry*
Tomography, Optical Coherence / methods*
Transfection / methods*

Substances

Ferric Compounds
ferric oxide
Gold