Exploring the Functional Disorder and Corresponding Key Transcription Factors in Intraductal Papillary Mucinous Neoplasms Progression

Guiying Bai; Chenxuan Wu; Yingtang Gao; Guiming Shu

doi:10.1155/2015/197603

Exploring the Functional Disorder and Corresponding Key Transcription Factors in Intraductal Papillary Mucinous Neoplasms Progression

Int J Genomics. 2015:2015:197603. doi: 10.1155/2015/197603. Epub 2015 Sep 3.

Authors

Guiying Bai¹, Chenxuan Wu², Yingtang Gao³, Guiming Shu⁴

Affiliations

¹ Department of Oncology, Tianjin Third Central Hospital, Tianjin 300179, China ; Key Laboratory of Artificial Cell Institute of Hepatobiliary Disease, Tianjin Third Central Hospital, Tianjin 300179, China.
² Department of Oncology, Tianjin Third Central Hospital, Tianjin 300179, China.
³ Key Laboratory of Artificial Cell Institute of Hepatobiliary Disease, Tianjin Third Central Hospital, Tianjin 300179, China.
⁴ Department of Hepatobiliary Surgery, Tianjin Third Central Hospital, Tianjin 300179, China.

Abstract

This study has analyzed the gene expression patterns of an IPMN microarray dataset including normal pancreatic ductal tissue (NT), intraductal papillary mucinous adenoma (IPMA), intraductal papillary mucinous carcinoma (IPMC), and invasive ductal carcinoma (IDC) samples. And eight clusters of differentially expressed genes (DEGs) with similar expression pattern were detected by k-means clustering. Then a survey map of functional disorder in IPMN progression was established by functional enrichment analysis of these clusters. In addition, transcription factors (TFs) enrichment analysis was used to detect the key TFs in each cluster of DEGs, and three TFs (FLI1, ERG, and ESR1) were found to significantly regulate DEGs in cluster 1, and expression of these three TFs was validated by qRT-PCR. All these results indicated that these three TFs might play key roles in the early stages of IPMN progression.